Abstract

Dendrobium officinale Kimura et Migo has been known for a long time as a precious orchid, which is used for medicine and functional foods that are widely commercialized in the world. The nodal explants could be obtained in high efficiency, good quality and uniform seedlings on multiplication vegetative in vitro. They can be propagated in large quantities in a short time. The studied results showed that the nodal explants grew on MS medium + 10 % coconut milk + 25 g sucrose + 0.5 mg/L BA + 6 g agar/ liter create high buds. This buds were used to A B 1 cm 1,5 cm create bud clusters with the aim of improving in vitro vegetative. The best bud culture medium for the formation of clusters was MS + BA 2.0 mg/L + NAA 0.4 mg/L; MS + kinetin 1.5 mg/L + NAA 0.3 mg/L; MS + TDZ 2.0 mg/L + NAA 0.4 mg/L; MS + adenine 1.5 mg/L + NAA 0.3 mg/L. However, the addition of kinetin or TDZ to the culture medium needed a steps to extend the bud subculture after 45 days in culture. The best medium for the rooting is MS + NAA 0.5 mg/L.